Journal: Materials science & engineering. C, Materials for biological applications

Article Title: Co-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineering

doi: 10.1016/j.msec.2019.01.127

Figure Lengend Snippet: SEM micrographs at lower magnification to provide a broad view of the scaffold (a) and at a higher magnification (b) of PCL and different cell-derived ECM PCL electrospun scaffolds fabricated. Fiber diameter distribution histograms (c) of PCL and the different cell-derived ECM PCL electrospun scaffolds. Scale bar 5 μm.

Article Snippet: Highlights Cell-derived extracellular matrix (ECM) as scaffolds for tissue engineering Mesenchymal stem/stromal cell and human umbilical vein endothelial cell ECM Electrospun polycaprolactone as a bone tissue engineering scaffolds Biomimetic microenvironment providing physical, chemical and mechanical cues for cells

Techniques: Derivative Assay

Journal: Materials science & engineering. C, Materials for biological applications

Article Title: Co-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineering

doi: 10.1016/j.msec.2019.01.127

Figure Lengend Snippet: Mechanical properties of cell-derived electrospun scaffolds obtained after tensile testing: Representative stress-strain curves (a), elastic modulus (b), ultimate tensile strength (UTS) (c) and ultimate elongation (d). Five different samples (N=5) were used in the analysis; * p < 0.05.

Article Snippet: Highlights Cell-derived extracellular matrix (ECM) as scaffolds for tissue engineering Mesenchymal stem/stromal cell and human umbilical vein endothelial cell ECM Electrospun polycaprolactone as a bone tissue engineering scaffolds Biomimetic microenvironment providing physical, chemical and mechanical cues for cells

Techniques: Derivative Assay

Journal: Materials science & engineering. C, Materials for biological applications

Article Title: Co-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineering

doi: 10.1016/j.msec.2019.01.127

Figure Lengend Snippet: Effects of cell-derived electrospun scaffolds on MSCs proliferation. Cell proliferation assay (a) and cell morphology assessment by DAPI-Phalloidin staining at day 21 (b). Values are expressed as mean ± SD (N=4); *p < 0.05, **p < 0.01, ***p < 0.001; Scale bar 100 μm.

Article Snippet: Highlights Cell-derived extracellular matrix (ECM) as scaffolds for tissue engineering Mesenchymal stem/stromal cell and human umbilical vein endothelial cell ECM Electrospun polycaprolactone as a bone tissue engineering scaffolds Biomimetic microenvironment providing physical, chemical and mechanical cues for cells

Techniques: Derivative Assay, Proliferation Assay, Staining

Journal: Materials science & engineering. C, Materials for biological applications

Article Title: Co-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineering

doi: 10.1016/j.msec.2019.01.127

Figure Lengend Snippet: Osteogenic differentiation of MSCs cultured on cell-derived ECM electrospun fibers. ALP activity (a) of MSCs cultured on cell-derived ECM PCL scaffolds after 14 and 21 days of osteogenic differentiation. Calcium deposition quantification (b) of MSCs seeded on cell-derived ECM PCL scaffolds after 14 and 21 days of osteogenic differentiation. Alizarin red, ALP and Von Kossa and Xylenol Orange stainings of MSCs differentiated on cell-derived ECM scaffolds after 21 days (c). Alizarin red staining confirmed the presence of calcium deposits (reddish areas). ALP/Von Kossa staining demonstrated ALP activity of MSCs cultured on all PCL scaffolds (reddish areas) and the presence of mineralized deposits (Von Kossa – darker areas, as highlighted by the white arrows). Xylenol Orange fluorescent staining confirmed the presence of calcium deposits. DAPI was used to counterstain the cell nuclei in blue. Scale bars, 200 μm. Values are expressed as mean ± SD (N=3); *p < 0.05.

Article Snippet: Highlights Cell-derived extracellular matrix (ECM) as scaffolds for tissue engineering Mesenchymal stem/stromal cell and human umbilical vein endothelial cell ECM Electrospun polycaprolactone as a bone tissue engineering scaffolds Biomimetic microenvironment providing physical, chemical and mechanical cues for cells

Techniques: Cell Culture, Derivative Assay, Activity Assay, Staining

Journal: Materials science & engineering. C, Materials for biological applications

Article Title: Co-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineering

doi: 10.1016/j.msec.2019.01.127

Figure Lengend Snippet: Morphology and elemental composition analysis of MSCs cultured on cell-derived ECM PCL electrospun fibers and PCL electrospun fibers after 21 days of osteogenic differentiation. SEM images and respective magnification (blank square) (a) and EDS spectrogram (b) for the different cell-derived ECM electrospun scaffolds. Scale bars, 10 μm.

Article Snippet: Highlights Cell-derived extracellular matrix (ECM) as scaffolds for tissue engineering Mesenchymal stem/stromal cell and human umbilical vein endothelial cell ECM Electrospun polycaprolactone as a bone tissue engineering scaffolds Biomimetic microenvironment providing physical, chemical and mechanical cues for cells

Techniques: Cell Culture, Derivative Assay

Journal: Materials science & engineering. C, Materials for biological applications

Article Title: Co-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineering

doi: 10.1016/j.msec.2019.01.127

Figure Lengend Snippet: Effects of cell-derived ECM electrospun scaffolds on ALP (a), Runx2 (b), OPN (c) and VEGF (d) gene expression by MSCs. Results are normalized to the endogenous control GAPDH and presented as fold change expression relative to MSC at day 0. Values are expressed as mean ± SD (N=3); *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Highlights Cell-derived extracellular matrix (ECM) as scaffolds for tissue engineering Mesenchymal stem/stromal cell and human umbilical vein endothelial cell ECM Electrospun polycaprolactone as a bone tissue engineering scaffolds Biomimetic microenvironment providing physical, chemical and mechanical cues for cells

Techniques: Derivative Assay, Gene Expression, Control, Expressing